Periodontal Diseases and Diabetes: Immunohistochemical Study and biological therapeutic Perspectives

Lucarini G*, Ciolino F, Zizzi A*, Aspriello S, Mattioli M, Lorenzini A, Orsini G, Putignano A, Piemontese M

*Dipartimento di Patologia Molecolare e Terapie Innovative, #Clinica Odontoiatrica

Obiejct: The association between diabetes mellitus (DM) and periodontal diseases has been extensively studied. Aim of the present study was an immunohistochemical investigation of VEGF, CD44 and CD133 expression in periodontal diseases of healthy (CTRL) and DM patients. Methods: 64 oral mucosa samples were considered: 16 healthy periodontal gingivae, as controls (CTRL), 16 diseased periodontal gingivae of patients without DM (DPG), 4 diseased periodontal gingivae of patients with type 1 DM (DPG-1DM) and 16 diseased periodontal gingivae of patients with type 2 DM (DPG-2DM). Gingival sections were incubated with the following monoclonal antibodies: anti-VEGF, anti-CD44 and anti-CD133 . The number of VEGF, CD44 and CD133 positive cells was counted among at least 1000 cells in the more representative fields. Data were subjected to one way analysis of variance and Bonferroni T test. Results: In diseased periodontal gingivae of patients with type 1 DM (DPG-1DM) a significant (p